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Image Search Results
Journal: Antioxidants
Article Title: Tetramethylpyrazine Analogue T-006 Protects Neuronal and Endothelial Cells Against Oxidative Stress via PI3K/AKT/mTOR and Nrf2 Signaling
doi: 10.3390/antiox13101272
Figure Lengend Snippet: Effects of T-006 in HT22 cells challenged by excessive glutamate. ( A ) Chemical structure of TMP and T-006. ( B ) Cell viability of HT22 cells treated with different concentrations of glutamate as measured by MTT assay. ( C ) Representative images of HT22 cells morphology under glutamate challenge (scale bar, 50 μm). ( D ) Schematic diagram of experiment protocol. HT22 cells were incubated with T-006 (1, 3, and 10 μM) or TMP (10 μM) for 24 h in the presence of glutamate. ( E ) Cell viability of HT22 cells treated with different concentrations of T-006 and TMP in the presence of glutamate (10 mM) as measured by MTT assay. Data are shown as mean ± SEM from at least three independent experiments (n > 3) and analyzed by one-way ANOVA followed by Tukey test. ### p < 0.001 vs. control group, *** p < 0.001 vs. glutamate-induced group.
Article Snippet:
Techniques: MTT Assay, Incubation, Control
Journal: Antioxidants
Article Title: Tetramethylpyrazine Analogue T-006 Protects Neuronal and Endothelial Cells Against Oxidative Stress via PI3K/AKT/mTOR and Nrf2 Signaling
doi: 10.3390/antiox13101272
Figure Lengend Snippet: T-006 inhibited glutamate-induced ROS production and apoptosis in HT22 cells. ( A , B ) Quantitative analysis of ROS generation by flow cytometry with H2DCFHDA. ( C , D ) Quantitative analysis of apoptosis by flow cytometry with Annexin V-FITC/PI. Data are shown as mean ± SEM from at least three independent experiments (n > 3) and analyzed by one-way ANOVA followed by Tukey test. ### p < 0.001 vs. control group, *** p < 0.001 vs. glutamate-induced group. “ns” stands for “not significant”.
Article Snippet:
Techniques: Flow Cytometry, Control
Journal: Antioxidants
Article Title: Tetramethylpyrazine Analogue T-006 Protects Neuronal and Endothelial Cells Against Oxidative Stress via PI3K/AKT/mTOR and Nrf2 Signaling
doi: 10.3390/antiox13101272
Figure Lengend Snippet: T-006 induced protective effect via AKT-mediated mTOR and HO-1 signaling in glutamate-challenged HT22 cells. ( A ) Representative immunoblots of AKT, mTOR, LC3, and p62 proteins. ( B – E ) Quantitative analysis for p-mTOR, p-AKT, LC3-I/II, and p62 proteins levels (n = 3–4). ( F ) Representative immunoblots of Nrf2 and HO-1 proteins. ( G , H ) Quantitative analysis for Nrf2 and HO-1 (n = 3–4). Data are shown as mean ± SEM and analyzed by one-way ANOVA followed by Tukey test. # p < 0.05 and ### p < 0.001 vs. control group; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. glutamate-induced group.
Article Snippet:
Techniques: Western Blot, Control
Journal: Antioxidants
Article Title: Tetramethylpyrazine Analogue T-006 Protects Neuronal and Endothelial Cells Against Oxidative Stress via PI3K/AKT/mTOR and Nrf2 Signaling
doi: 10.3390/antiox13101272
Figure Lengend Snippet: T-006 induced protective effects in glutamate-challenged HT22 cells was inhibited by LY294002. ( A ) Representative images of cell morphology with bright field microscope (scale bar 100 μm). ( B ) Cell viability was detected by MTT assay (n = 6). ( C ) Representative immunoblots of AKT and mTOR proteins. ( D , E ) Quantitative analysis for p-mTOR and p-AKT (n = 3–4). ( F ) Representative immunoblots of Nrf2 and HO-1 proteins. ( G , H ) Quantitative analysis for Nrf2 and HO-1 (n = 3–4). Data are shown as mean ± SEM and analyzed by one-way ANOVA followed by Tukey test. # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. control group; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. glutamate-induced group. “ns” stands for “not significant”.
Article Snippet:
Techniques: Microscopy, MTT Assay, Western Blot, Control